| abstract |
The present invention relates to a kind of test card, preparation and its detection method for detecting transmissible gastro-enteritis virus antibody, belong to field of immunological detection, test card includes getting stuck and test strips, and test strips include bottom plate and overlap joint is pasted onto water absorption pad, detecting pad, bonding pad and sample pad on bottom plate successively；Detecting pad is the nitrocellulose filter equipped with nature controlling line C and detection line T, and nature controlling line C is coated with goat-anti mouse monoclonal antibody, the transmissible gastro-enteritis virus of detection line T coating inactivations；The glass fibre element film for the anti-E2 protein monoclonal antibodies that bonding pad marks for embedding time-resolved fluorescence microballoon；Sample pad is dry glass fibre element film after sample treatment liquid immersion treatment.Test card prepared by the present invention has the sensitivity of more preferable stability, higher, while can be analyzed by fluorescence signal and achieve the purpose that half-quantitative detection. |