http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107916271-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-01084 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2017-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107916271-B |
| titleOfInvention | A kind of high-efficiency expression method of recombination nitrile hydratase |
| abstract | The invention discloses a kind of high-efficiency expression method of recombination nitrile hydratase, this method includes：(1) clone obtains target gene from original strain；(2) recombinant vector is built, recombinant vector is converted to host cell, genetic engineering bacterium is obtained；(3) genetic engineering bacterium is cultivated, recombination nitrile hydratase albumen is obtained；The target gene is connected in sequence by the nitrile hydratase gene and activation elements gene of coding for alpha subunit and β subunits；Wherein, the base sequence of the nitrile hydratase gene is as shown in SEQ ID NO.1 or 4, and the base sequence of the activation elements gene is as shown in SEQ ID NO.2 or 5.The method of the present invention uses self-induction medium culture engineering bacteria by the way that activation elements gene is added on the basis of nitrile hydratase structural gene, obtains the recombination nitrile hydratase of high vigor. |
| priorityDate | 2017-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.