http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107904257-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-11002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-12 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2017-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107904257-B |
| titleOfInvention | Method for increasing protein expression quantity of CHO cell group |
| abstract | The invention discloses a method for increasing the protein expression quantity of a CHO cell group, which controls the transcription of key enzymes on a lactic acid metabolic pathway by utilizing a shRNA technology and comprises the following specific steps: 1) constructing a plasmid containing a protein sequence to be expressed and a plasmid containing shRNA for expressing key enzyme on a lactic acid metabolic pathway; 2) introducing the plasmid constructed in the step 1) into CHO cells by a transfection method; 3) screening antibiotics for the cells transfected in the step 2); 4) after the cell state is recovered, adopting the cells screened in the step 3) to produce target protein; the key enzymes on the expression lactate metabolic pathway comprise lactate dehydrogenase and pyruvate dehydrogenase kinase. Compared with the traditional method that the pH needs to be closely monitored and continuously adjusted by adding alkali in CHO cell mass production, the method provided by the invention only additionally and jointly transfects a plurality of shRNA plasmids during transfection, not only greatly reduces the operation complexity, but also greatly guarantees the reduction of lactic acid and the improvement of final protein yield. |
| priorityDate | 2017-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.