http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107904215-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16151 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
| filingDate | 2017-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107904215-B |
| titleOfInvention | Full suspension culture method of avian influenza virus |
| abstract | The invention discloses a full suspension culture method of avian influenza virus, which comprises the following steps: step 1: EB66 cells are taken for growth culture; step 2: when the density of EB66 cells grows to be suitable for virus inoculation, adopting a fed-batch fluid infusion virus inoculation process to inoculate the avian influenza virus into EB66 cells, and carrying out virus propagation culture; and step 3: sampling every 12h after inoculation for 24h to determine the HA titer of the virus, and harvesting and storing the virus when the HA titer of the virus reaches the highest value to obtain the cultured avian influenza virus. According to the invention, the EB66 cells are used for culturing the avian influenza virus, so that the defect that a large amount of foreign proteins are easily introduced when the traditional chick embryo culture process is adopted for production is avoided, the occurrence of immune side reactions of chickens is effectively reduced, the titer and purity of the cultured avian influenza virus are improved, and the production quality of the avian influenza vaccine is further improved. |
| priorityDate | 2017-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.