| abstract |
The invention discloses a kind of hybridizing tumour cell non-serum culture medium, including the following raw material：Alanine, arginine, aspartic acid, N, cystine, cysteine, glutamine, glutamic acid, glycine, histidine etc., calcium chloride, magnesium sulfate, potassium chloride, potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium acid carbonate, sodium hydroxide, green vitriol, Magnesium dichloride hexahydrate, copper sulphate, Sodium Pyruvate, manganous chloride, sodium selenite, ammonium molybdate etc., niacinamide, p-aminobenzoic acid, folic acid, ribose, uracil, lipoic acid, linoleic acid L, cholesterol, dexamethasone, estradiol progesterone, testosterone etc..Culture medium of the present invention, has been entirely free of serum composition；The antibody purity of production increases substantially；The culture medium has high amplification rate and high antibody expression ability simultaneously；The culture medium can keep cytoactive for a long time, and the Hybridoma Cell Culture time greatly prolongs, and can greatly prolong the production time, improve antibody production. |