| abstract |
The invention discloses a kind of store method for turning streptococcus suis 2-type mrp gene engineering bacteria, it is related to gene engineering technique field.The store method disclosed by the invention for turning streptococcus suis 2-type mrp gene engineering bacteria thinks the physiological saline for containing glycerine as preservation reagent, reagent will be preserved to add into the bacterium solution containing object bacteria, it is sub-packed in sterilized EP pipes, and Cord blood is carried out under the conditions of being immediately placed on 80 DEG C~20 DEG C；The store method is simple to operate, the resting period is long, occupy little space, cost is cheap, once freezes multitube dispenses in a small amount, convenient management, prevents multigelation from causing thalline death and living contaminants, improves thalline survival rate；And by prolonged preservation remain in that with primary strain identical vigor and purity, important preservation is played to the stability for maintaining strain and plasmid and acted on. |