http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107858384-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0016 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01009 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y102-01002 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
filingDate | 2017-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107858384-B |
titleOfInvention | Method for preparing optically pure L-tert-leucine by using active inclusion bodies |
abstract | The invention discloses a method for preparing optically pure L-tert-leucine by using active inclusion bodies, which comprises the following steps: (1) preparing a bifunctional enzyme active inclusion body, wherein the active component of the bifunctional enzyme active inclusion body is a fused bifunctional enzyme, and the fused bifunctional enzyme comprises a leucine dehydrogenase (LeuDH) part and a NAD (nicotinamide adenine dinucleotide) part for coenzyme which are connected by a connecting peptide + A regenerated polymerase portion; (2) adding the bifunctional enzyme activity inclusion body into a reaction mixed solution with the pH of 6.0-10.0 for resuspension, and then reacting at 20-40 ℃, wherein the pH is controlled to be 6.0-10.0 during the reaction; the reaction mixture comprises 50-1000mM trimethylpyruvic acid, 50-1000mM ammonium formate and 0.05-5 mM coenzyme NAD + 。 |
priorityDate | 2017-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 664.