http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107858331-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-727 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-385 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0618 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0797 |
| filingDate | 2017-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107858331-B |
| titleOfInvention | Method for inducing differentiation of human pluripotent stem cells into spinal cord motor nerve precursor cells |
| abstract | The invention discloses a method for inducing human pluripotent stem cells to differentiate into spinal cord motor nerve precursor cells, which comprises the following steps: 1) culturing human pluripotent stem cells: digesting human pluripotent stem cells into single cells, inoculating the single cells into a coated culture dish for adherent culture, wherein the culture solution is pluripotent stem cell culture solution and 5% CO at 37 DEG C 2 Culturing in a saturated humidity incubator for 20-28 hr; 2) differentiation induction of spinal motor precursor cells: culturing the human pluripotent stem cells obtained in the step 1) in a basic complete culture medium, adding different space-time specific signal paths at different times to regulate and control small molecules and/or growth factors to induce differentiation, changing the medium once every two days, and changing the medium at 37 ℃ by 5% CO 2 Culturing in saturated humidity incubator for 8-20 days. The method is efficient, rapid, stable, safe and simple and convenient to operate, and more than 90% of spinal cord motor nerve precursor cells can be obtained on the eighth day of differentiation. |
| priorityDate | 2017-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 160.