http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107828762-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2437 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01004 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14 |
filingDate | 2017-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107828762-B |
titleOfInvention | Endoglucanase, its encoding gene cel5A-h50 and its application |
abstract | The invention belongs to the field of genetic engineering, and in particular relates to an endoglucanase, its encoding gene cel5A-h50 and applications thereof. A gene cel5A-h50 encoding the endoglucanase, the nucleotide sequence of which is shown in SEQ ID No.1. Bioinformatics analysis showed that the endoglucanase encoded by this gene belongs to the fifth family of glycoside hydrolases. The inventors successfully cloned it by designing primers, and successfully expressed it in a prokaryotic expression system. The optimum pH of the recombinant endoglucanase purified by induction ultrasonication was 5.0, and the optimum temperature was 45°C. Relatively stable enzyme activity can be maintained below °C, with strong pH tolerance at pH 5.0-8.0, and high enzyme activity can be maintained. The recombinant endoglucanase has great research and industrial application potential. |
priorityDate | 2017-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 396.