http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107828709-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-0313 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1085 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y205-01021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y504-99017 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-02 |
| filingDate | 2017-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107828709-B |
| titleOfInvention | Recombinant escherichia coli for heterologous synthesis of ambergris alcohol and construction method thereof |
| abstract | The invention discloses a recombinant escherichia coli for heterologously synthesizing ambergris alcohol and a construction method thereof, and the method comprises the following steps: fusing saccharomyces cerevisiae squalene synthase gene ERG9 with 26 amino acid residues at the truncated C end and the upstream and downstream homologous arms of lacZ locus of escherichia coli into donor DNA; constructing a plasmid 1; transforming the donor DNA and the plasmid 1 into escherichia coli together, eliminating the plasmid 1 to obtain recombinant escherichia coli for synthesizing squalene, namely a strain 1; the acid-heated alicyclic acid bacillus squalene-hopene cyclase gene D377C SHC after the 377 th amino acid residue is mutated into a cysteine residue and the bacillus megaterium cyclase gene BmeTC are connected into a segment and then inserted into an escherichia coli expression plasmid p5C to obtain a plasmid 4; thirdly, transforming the plasmid 4 into the strain 1 to obtain recombinant escherichia coli for heterologously synthesizing ambergris alcohol, namely the strain 4; experiments prove that the ambergris alcohol is obtained by fermenting recombinant escherichia coli for heterogeneously synthesizing ambergris alcohol. |
| priorityDate | 2017-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 72.