http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107805627-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70596 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2017-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107805627-B |
| titleOfInvention | Construction method of hESC indicating cell line for specifically tracing endothelial cell differentiation |
| abstract | The invention discloses a method for constructing an hESC (human embryonic stem cell) indicating cell line for specifically tracing endothelial cell differentiation, which comprises the following steps: (1) constructing a plasmid CD 144N-GFP; (2) constructing plasmids TALENs which comprise plasmids CD144N-L2 and plasmids CD 144N-R2; (3) the plasmid CD144N-GFP and the plasmid TALENs are simultaneously subjected to nuclear transfer of ESCs, after G418 screening and single cloning, PCR preliminary identification is carried out by using a 5 'spanning homology arm primer F1/R1 and a 3' spanning homology arm primer F2/R2, Southern blot identification is carried out on double-side positive persons, Kpn I enzyme digestion is carried out, hybridization identification is carried out on a probe on a long homology arm, and the positive persons are hESC indicating cell lines for specifically tracing endothelial cell differentiation. |
| priorityDate | 2017-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.