abstract |
Application the present invention relates to aspergillus niger yy07 bacterial strains and its in solid fermentation produces feeding acid protease, can effectively solve the problems, such as to produce feeding acid protease, by aspergillus niger( Aspergillus niger )Yy07, which is inoculated into slant medium, carries out amplification cultivation, 70 75h are cultivated at 35 40 DEG C, it is placed in inclined-plane isolation medium, mycelia covers with inclined-plane isolation medium, as slant medium seed, then it is inoculated in solid fermentation culture medium, in 35 40 DEG C of cultures, treat that mycelia is covered with media surface, untill spore whole blackening, it is then seeded into solid fermentation culture medium, cultivated at 35 40 DEG C, when black spore will be changing into by staying in the white hypha grown on culture medium, it is dried less than 60 DEG C to moisture content below 10%, crush, the present invention is effective for producing feeding acid protease, its preparation method science, it is advanced, abundant raw material, cost is low, energy-conservation, environmental protection, easy popularization and application, economic and social benefit is huge. |