http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107759684-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-57581 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-575 |
filingDate | 2016-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107759684-B |
titleOfInvention | Recombinant periplaneta americana thymosin protein PaTHY1 and expression method thereof |
abstract | The invention relates to the technical field of biology, and discloses a recombinant periplaneta americana thymosin protein PatHY1 and an expression method thereof, wherein the method comprises the following steps: (1) extracting RNA from periplaneta americana tissues and carrying out reverse transcription to obtain cDNA; (2) synthesizing a primer: (3) obtaining a periplaneta americana thymosin protein coding gene fragment by using the total cDNA as a template through PCR; (4) cloning the coding gene into a prokaryotic expression vector, transforming host bacteria, culturing the obtained recombinant engineering bacteria, and then inducing the recombinant engineering bacteria to express a target protein; (5) and purifying the target protein by adopting an affinity chromatography method so as to obtain the recombinant periplaneta americana thymosin protein PatHY 1. The recombinant protein is obtained by gene engineering for the first time, solves the problems of extremely low content of thymosin in complex chemical components of the periplaneta americana and difficulty in purification, and lays a foundation for the research on pharmacodynamic functions of the thymosin. |
priorityDate | 2016-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 177.