http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107702959-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-305 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
filingDate | 2017-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107702959-B |
titleOfInvention | Paraffin section method for gradient dehydration of plant material |
abstract | The invention discloses a paraffin sectioning method for gradient dehydration of plant materials, which comprises the steps of sample collection and fixation, sample dehydration, sample transparency, wax immersion, embedding, block trimming, fixation, slicing, sheet unfolding, drying, dewaxing, sealing and the like. The sample material of the invention is transparent by xylene, although chlorophyll in plant body can be completely degraded, cell wall and cell nucleus of plant cell are dyed red by safranin, so that the plant sample presents red color, after the sample material is transparent by xylene, the color of the sample and the color of embedded paraffin are easy to distinguish, the sample is easy to be embedded into the required sample direction when the sample is embedded, and the position of the material in the embedded block can be clearly seen when the block is repaired, so that the operations of repairing the block, slicing, spreading and microscopic examination are very simple and convenient, the material can be prevented from being accidentally injured, the expansion degree of the material can be conveniently detected when the microscopic examination is carried out, and further the slicing quality and the experimental efficiency are improved. |
priorityDate | 2017-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.