http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107699550-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate | 2017-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107699550-B |
| titleOfInvention | A group of Burkholderia homologous recombinases and their expression vectors and applications |
| abstract | The invention discloses a group of homologous recombinases capable of efficiently mediating recombination in Burkholderia, including a protein Redα7029 with exonuclease function and a protein Redβ7029 with single-chain annealing protein function. The present invention also discloses an expression vector pBBR1-Rha-BA 7029-Km containing the homologous recombinase, and the homologous recombinase is combined with the double-stranded DNA exonuclease inhibitory protein Redγ derived from E. coli The application in improving the recombination efficiency in Burkholderia and the application of the homologous recombinase in obtaining the compound glidopeptin or rhizopeptin. The present invention can be used to iteratively delete multiple genes so as to realize the genome reduction of Burkholderia, or insert functional promoters to activate unknown biosynthetic gene clusters in situ, etc., for bioprospecting and functional genomics research of Burkholderia. have important meaning. |
| priorityDate | 2017-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 85.