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filingDate 2017-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107699535-B
titleOfInvention Recombinant bacillus subtilis for induced synthesis of guanosine diphosphate fucose and construction method and application thereof
abstract The invention provides a recombinant bacillus subtilis for induced synthesis of guanosine diphosphate fucose, a construction method and application thereof, wherein the recombinant bacillus subtilis is obtained by induced expression of a sugar transporter gene of bacillus subtilis 168 and expression of exogenous fucokinase and phosphoguanyltransferase genes. The invention enhances the reaction from pyruvic acid to oxaloacetic acid, increases the carbon flux flowing to oxaloacetic acid, enhances the transportation of fucose by cell membranes, increases the concentration of intracellular fucose and promotes the synthesis of guanosine diphosphate fucose. The recombinant bacillus subtilis has a simple construction method, is convenient to use and has a good application prospect.
priorityDate 2017-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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