http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107698676-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-59 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-59 |
| filingDate | 2017-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107698676-B |
| titleOfInvention | Method for extracting and preparing high-purity menotrophin |
| abstract | The invention provides a preparation method of high-purity menotrophin, which comprises the following steps of (1) purifying a crude product of human menopausal gonadotropin by anion exchange chromatography to obtain a high intermediate I, (2) purifying the intermediate I by cation exchange chromatography to obtain an intermediate II, and (3) purifying the intermediate II by dye affinity chromatography to obtain the high-purity menotrophin, wherein the invention adopts the processes of anion exchange chromatography, cation exchange chromatography and dye affinity chromatography in sequence to extract and purify to obtain the high-purity HMG, the purification multiple of the HMG can reach more than 60 times by the anion exchange chromatography and the cation exchange chromatography, the impurity protein is further removed by utilizing the affinity adsorption property of FSH and L H and the dye, and the purification multiple of the HMG can reach more than 500 times by the 3-step chromatography process to obtain the high-purity menotrophin, wherein the ratio of FSH to L H is about 1:1. |
| priorityDate | 2017-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.