http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107674859-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-727 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-235 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0655 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
| filingDate | 2017-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107674859-B |
| titleOfInvention | Method for inducing mouse fibroblast to form cartilage by using small molecule composition |
| abstract | The invention discloses a method for inducing mouse fibroblast to form cartilage by using a small molecule composition, which comprises the following steps: removing the culture medium after adherent culture of mouse embryonic fibroblasts, slowly adding a chemical induction culture medium containing the small molecule composition, culturing at 37 ℃ in an environment with 3-8% of oxygen, 3-8% of carbon dioxide and the balance of nitrogen, and replacing the chemical induction culture medium containing the small molecule composition once every 2-3 days; after continuously culturing for 4-12 days, obtaining intermediate cells; the small molecule composition comprises: HDAC inhibitors, GSK-3 inhibitors and TGF-beta signaling pathway inhibitors; transferring the intermediate state cells to a cartilage induction culture medium, culturing at 37 ℃ in an environment with 15-25% of oxygen, 3-8% of carbon dioxide and the balance of nitrogen, replacing a fresh cartilage induction culture medium once every 3-4 days, and culturing for 14-28 days to obtain a cartilage cell mass; the method gets rid of the method that the fibroblast can be induced to transdifferentiate into the chondrocyte only by introducing the exogenous gene, and is expected to further solve the problems of chondrocyte seed cell deficiency or in-situ focus fibrosis. |
| priorityDate | 2017-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 373.