| abstract |
The one tobacco articles in vitro toxicology evaluation preparation method of three-dimensional cell bracket, it is characterized in: is polymerize in Tissue Culture Dish by High Internal Phase Emulsion, it obtains a kind of with polymer intercommunication porous material, material has tobacco product in vitro toxicology evaluation cell (human lung carcinoma cell, human bronchial epithelial cell) size macropore, it and is intercommunication between Kong Yukong, oxygen needed for being grown conducive to cell, the input of nutriment and the output of cell metabolism waste, material surface is modified and makes it have good cellular affinity, compared with existing toxicological evaluation generallys use plate monolayer cell culture method, cell can on bracket of the invention three dimensional growth, solid shape and biochemical and functionality when in substance are more nearly, therefore, the external toxicity of tobacco product is carried out using the cell of three-dimensional cell bracket culture of the invention When learning evaluation, evaluation result will be more objective, true.And the cytoskeleton is prepared in culture dish, it is more convenient to subsequent experimental. |