http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107646681-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2017-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107646681-B |
| titleOfInvention | method for improving rapid seedling formation of barley tissue culture |
| abstract | the invention provides a method for improving rapid seedling formation of barley tissue culture, which comprises the following steps: 1) selecting barley young embryos with the diameter of 1-3cm, and respectively using alcohol and sodium hypochlorite to disinfect the surfaces of the barley young embryos to reduce the contamination rate of mixed bacteria; 2) accurately separating the coleoptile of the young embryo, carrying out secondary treatment on the scutellum, and inducing the callus to rapidly generate; 3) adjusting the time of pre-culture and agrobacterium infection according to the size of the immature embryo to prevent the bacterial liquid from being excessive; 4) the green point differentiation rate and the seedling rate are improved by adopting a weak light shielding method; 5) and adjusting the antibiotic ratio and the use time of the callus induction culture medium, the differentiation culture medium and the rooting culture medium to induce the callus to quickly grow seedlings. The invention is a tissue culture method of barley embryo, the callus generation is fast, the embryogenic callus is more, the operability is strong, the green point differentiation and seedling rate are high, and the method is not limited to the restriction of material genotype; the established transformation and regeneration system of the barley immature embryo can provide technical support for exploring the research of barley genetic improvement and cell engineering breeding. |
| priorityDate | 2017-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.