| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_805c4456058924190338e401a650d3f0 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10352
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10343 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-235
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate |
2017-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68b80a52e3c359d4abbca1e5fcf2614b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c52c89aa97a0339b8c7ce5d2fb85305
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cca0266dec4107c164cb11cd85f9ed37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2e87a762fece6a713eb3193eb0c7b8f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd64b2b62290555eb7e1530a586dd168
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5768e943e69283e13b72cae52f3813f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8942d09d5077a0ef4d29ddce54ddfbfc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_233dc355301fb0ba197805116a6405eb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_609cf08255bb4c3a918452d4d8b9b939
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f79aa0302f381b75a50144d65254da11 |
| publicationDate |
2018-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-107630004-A |
| titleOfInvention |
Cell line, construction method and application of reduced replicable adenovirus production |
| abstract |
The present invention provides a cell line that reduces the production of replicable adenovirus and its construction method and application. The cell line HEK293.CS that reduces the production of replicable adenovirus is obtained by knocking out the gene fragment homologous to Ad5 adenovirus E1 gene in HEK293 , while providing a template plasmid to replace it with a non-homologous sequence that can stabilize the expression of the E1 gene, a safe adenovirus production cell line was constructed. Compared with the unmodified HEK293 cell line, the growth ability and production capacity of HEK293.CS The virus ability is not reduced, but no detectable RCA is produced; the cell line can be used for large-scale culture of recombinant human adenovirus type 5, and reduces the probability of RCA production in the process of manufacturing vaccines, antibodies and other drugs. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019042307-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115232835-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108456696-A |
| priorityDate |
2017-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |