http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107574207-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L33-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-01 |
| filingDate | 2016-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107574207-B |
| titleOfInvention | Preparation method of homoarginine mixed peptide and application of homoarginine mixed peptide in colorectal cancer treatment |
| abstract | The invention relates to a mixed peptide with high arginine content and application thereof in colorectal cancer treatment. The preparation method comprises the following steps: pretreating the walnut pulp and egg white protein suspension by using ultrahigh pressure, and then performing stepwise enzymolysis on pretreated products by using alkaline protease and papain under the auxiliary action of ultrasonic waves and microwaves; separating the target mixed peptide in the clear liquid after the enzymolysis liquid is filtered by a reversed-phase high performance liquid chromatography; the mixed peptide is used as a template molecule, acrylic acid and methacrylic acid are used as functional monomers, triethylene glycol dimethacrylate is used as a cross-linking agent, isopropyl thioxanthone is used as an initiator, and ultraviolet initiation polymerization is carried out to form a surface molecularly imprinted membrane which is used for separating and enriching target mixed peptide in enzymolysis clear liquid. The content of arginine in the mixed peptide is more than 18 percent; the MTT detection shows that the homoarginine mixed peptide can effectively inhibit the proliferation of LS174T cells of human colorectal cancer. The method is helpful for reducing the product cost and accelerating the realization of industrial production. |
| priorityDate | 2016-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 89.