http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107574160-A

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assignee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1be831199383dca13e878cb89c323482
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-32
filingDate 2017-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e7ed29798fdd3dce3912ef5930d5795
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9ba22d623293623d0d9e8e8ccae9b68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e412779f43f7739b0d93da84736e774
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publicationDate 2018-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107574160-A
titleOfInvention A kind of method for producing alpha amylase
abstract The invention discloses a kind of method for producing alpha amylase, weighs the wheat seed crushing of firm rudiment, absolute ethyl alcohol is added into wheat seed, and after 1 5h of immersion, centrifugation obtains the wheat seed crossed through soaked in absolute ethyl alcohol;Ethanol water is added into the wheat seed crossed through soaked in absolute ethyl alcohol, is then centrifuged after the 5h of ultrasonic extraction 2, obtains supernatant;Acetone, chloroform, n-butanol are added into supernatant, is then extracted, obtains raffinate A;3 5h are stood after xanthans, potassium permanganate, ethanol, methanol, sodium-acetate buffer are well mixed, mixed liquor A is made;Mixed liquor A and raffinate A are mixed into 1 6h, is then extracted, obtains raffinate B;Extract B is dried in vacuo, produces alpha amylase.The present invention has extracted alpha amylase by ultrasonic extraction and screening from the wheat seed of firm rudiment, and preparation technology is simple, and easily controllable, realization, production cost is low, can effectively improve economic benefit.
priorityDate 2017-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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