http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107557358-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2017-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107557358-B |
| titleOfInvention | VHH-ELISA kit suitable for fipronil residue analysis |
| abstract | The invention discloses an ELISA kit for detecting fipronil residue based on a nano antibody and application thereof, wherein the kit comprises a kit body, an ELISA plate and a reagent, wherein the ELISA plate and the reagent are arranged in the kit body; the reagent comprises an anti-fipronil nano antibody, a fipronil standard solution, an enzyme-labeled secondary antibody, a buffer solution PBS, a washing solution PBST, a substrate solution, a developing solution, a reaction stop solution and the like. In the detection process, the coating antigen adsorbed on the pore wall of the ELISA plate and the fipronil to be detected compete with each other to react with the antibody, and the result is observed through a color reaction. The invention also provides a C-terminal universal PCR primer for constructing the camelid phage display nano antibody library. The method has the advantages that the method can accurately and sensitively detect the fipronil residues in water and vegetables, the pretreatment process of the sample is simple, the consumed time is less, a large amount of samples can be detected simultaneously, and the sample detection cost is far lower than that of the traditional instrument detection method. |
| priorityDate | 2017-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 140.