http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107541482-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2016-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107541482-B |
| titleOfInvention | Method for constructing escherichia coli high-efficiency secretory expression transpeptidase Sortase A |
| abstract | The invention discloses a method for constructing escherichia coli high-efficiency secretory expression transpeptidase sortaseA, belonging to the field of genetic engineering. The invention successfully realizes the high-efficiency secretory expression of the sortaseA in the escherichia coli and solves the problems that the enzyme cannot be secreted and expressed and the intracellular expression level is low in the existing report. In a shake flask level, the yield of the obtained Sortase A can reach 90.2mg/L, and the level of the extracellular enzyme activity can reach 34.2U/mL; lays a foundation for the subsequent industrial production of the Sortase A. |
| priorityDate | 2016-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.