http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107529549-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2017-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107529549-B |
| titleOfInvention | Method for visually detecting target protein expression and purification by utilizing cyanobacterial flavin protein FNR |
| abstract | The invention discloses a method for visually detecting target protein expression and purification by utilizing cyanobacteria flavoprotein FNR, which comprises the steps of firstly constructing a fusion gene of a target protein gene and a cyanobacteria FNR gene or a homologous gene thereof, transferring an expression vector containing the fusion gene into an expression strain for expression, judging whether the fusion protein is successfully expressed and/or purified by observing the color of a supernatant and/or a purified component thereof obtained after crushing an expression bacterial liquid, and indicating that the target protein and the cyanobacteria FNR protein or the homologous protein thereof are expressed and/or purified together if the supernatant and/or the purified component are yellowish. The method realizes the visual detection of the expression and purification of the target protein, and is convenient and rapid. |
| priorityDate | 2017-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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