http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107513519-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-392 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-135 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-1369 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0622 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-079 |
| filingDate | 2017-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107513519-B |
| titleOfInvention | Culture method of Schwann cells |
| abstract | The invention discloses a culture method of Schwann cells, which comprises the following steps: s1, culturing the cord blood mononuclear cells by using a MSCs culture medium to obtain P1 generation cord blood MSCs; s2, culturing the P1 generation cord blood MSCs in a neurosphere culture medium to obtain P3 generation neurospheres; and S3. culturing the P3 generation neurosphere in a Schwann cell culture medium to obtain Schwann cells. The invention has the beneficial effects that: the culture method of the Schwann cells is a method for culturing the Schwann cells derived from cord blood MSCs through a nestin + neurosphere stage. The P1 generation cord blood MSCs are adopted to induce the formation of neurospheres, and the neurosphere formation rate is as high as 41%. The invention also provides a specific neurosphere culture medium which is enough to induce the MSCs of the umbilical cord blood to form nestin + neurospheres; provides a specific culture scheme for differentiation from nestin + neural precursor cells to schwann cells, can remarkably promote the attachment and migration of nestin + neural precursor cells, and prevent agglomeration and multilayer growth in the cell proliferation process. |
| priorityDate | 2017-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.