http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107502606-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2017-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107502606-B |
| titleOfInvention | Method for extracting endotoxin-removing plasmid in large scale |
| abstract | The invention relates to a method for extracting endotoxin-removing plasmid in large scale, which comprises the steps of obtaining roughly separated plasmid DNA by a classical alkali cracking method, and then sequentially adding isopropanol and NH 4 Ac solution, RNase A and NaCl, and then PEG6000 and NaC are addedAnd l, dissolving the precipitate by using a TE solution during the period, re-precipitating by using absolute ethyl alcohol, washing the precipitate by using 70% ethyl alcohol, matching with a centrifugal operation, drying, and finally dissolving the precipitate by using the TE solution to obtain the target plasmid. The method can well remove the pollution of endotoxin on plasmid DNA, all reagents used in the method are chemical reagents which are commonly prepared in laboratories, and the method is simple in operation, easy to master and suitable for large-scale plasmid extraction. The concentration of the plasmid obtained by the invention can reach 5mg/mL, the endotoxin is lower than 5 EU/mug, and the plasmid can be used for enzyme digestion, DNA sequence sequencing, cell transfection, virus packaging and clinical animal immunity experiments. |
| priorityDate | 2017-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.