| abstract |
The invention discloses a concentration gradient rhIL-2-dependent iNKT cell expansion method and application thereof. The method includes (1) extracting peripheral blood mononuclear cell PBMC; adding a final concentration of 100ng/ml α-GalCer to the PBMC cells extracted in step (1) for 48 hours; Increase the concentration of rhIL-2 in the medium. In the invention, iNKT cells are harvested on the 21st day of culture, the number of iNKT cells is increased by 100,000 times, and more than 90% of the iNKT cells are CD4-iNKT cells, which can upregulate immune response and directly kill tumors. The iNKT cells activated and amplified by the method of the present invention have higher amplification efficiency, selectively amplify Th1-like iNKT cells, and strengthen the functions of immune enhancement, tumor immune monitoring and killing of iNKT cells amplified in vitro. |