http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107502588-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0664 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0226 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0215 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
| filingDate | 2017-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-04-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107502588-B |
| titleOfInvention | Method for separating and preparing dental pulp stem cells |
| abstract | The invention relates to the technical field of cell culture, in particular to a method for separating and preparing dental pulp stem cells. The natural compound nitidine chloride is used as the bacteriostatic agent in the preparation process of the dental pulp stem cells, so that the dental pulp stem cells have high safety, good stability and obvious bacteriostatic effect, can effectively reduce the infection of bacteria, fungi or mycoplasma, and are superior to the traditional method of simply using antibiotics. In the culture process, the culture dish is coated with hyaluronic acid and gelatin, so that the number of adherent dental pulp stem cells is increased, the growth of the cells is promoted, and the aim of effective amplification is fulfilled. Experiments show that the method provided by the invention has the advantages that the cell quantity obtained by amplifying the dental pulp mesenchymal stem cells is large, the pollution rate is low, the detection of the surface markers of the obtained dental pulp stem cells meets the standard, the positively expressed surface markers are more than or equal to 95 percent, and the negatively expressed surface markers are less than or equal to 2 percent. |
| priorityDate | 2017-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.