http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107496457-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-1358 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-083 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-062 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0663 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-079 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0793 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2017-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107496457-B |
| titleOfInvention | Method for growing OPCs (OpCs) trophoblastic sensory neuron axons from MSCs (mesenchymal stem cells) |
| abstract | The invention discloses a method for growing axons of trophoblastic sensory neurons by OPCs (optical fiber) from MSCs (mesenchymal stem cells), which comprises the following steps of: s1, culturing neonatal umbilical cord Wharton jelly in a MSCs culture medium to obtain P2 umbilical cord MSCs; s2, suspending the P2 umbilical cord MSCs by using an MSCs culture medium, inoculating the MSCs into a cell culture plate coated by the recombinant human laminin and the recombinant human vitronectin, culturing to 60-70% confluence, and replacing a neural differentiation culture medium to prepare neural epithelial pre-induced cells; s3, culturing the neural epithelial pre-induced cells in an OPCs culture medium to obtain P0 generation OPCs, and subculturing to P2 generation OPCs; s4, preparing OPCs trophoblasts by using P2 generation OPCs; s5, preparing DRG sensory neuron cells, and co-culturing OPCs trophoblasts and the DRG sensory neuron cells. The method for nourishing and sensing neuron axon growth by OPCs from MSCs provides a new idea for cell therapy of central nervous system nerve injury repair. |
| priorityDate | 2017-08-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 294.