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filingDate 2017-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27b32e61e0b004373173b92f7f8d5f48
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publicationDate 2017-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107494270-A
titleOfInvention The tissue culture and rapid propagation method of gold leaf common bluebeard
abstract The invention discloses a kind of tissue culture and rapid propagation method of gold leaf common bluebeard, including:Sterilization:Using the stem section of gold leaf common bluebeard as explant, carry out disinfection sterilizing;Initial culture:Explant is inoculated in Initial culture base and cultivated, obtains sprout;The induction of callus:Sprout is taken to be cut into stem section, several knives of rip cutting are followed by cultivating in inducing culture on the surface, obtain callus;Differentiation culture:Callus is accessed in differential medium and cultivated, obtains budlet;Strong seedling culture:Budlet is accessed into strong seedling culture base culture, obtains unrooted seedling;Culture of rootage:Unrooted seedling is accessed into root media culture;Hardening and transplanting:Culture of rootage for a period of time after acclimatization and transplantses.The present invention is from the sterilization of explant, Initial culture, the induction of callus, differentiation culture, strong seedling culture, culture of rootage and the hardening of tissue-cultured seedling and transplanting etc. progress more systematic research, gold leaf common bluebeard's seedling quickly to breed excellent provides technical support, meets increasingly demand of the market to gold leaf common bluebeard.
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type http://data.epo.org/linked-data/def/patent/Publication

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