http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107490694-A

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publicationDate 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107490694-A
titleOfInvention A kind of method of TEM type beta lactamases in detection breast using colloidal gold immuno-chromatography test paper strip
abstract The invention discloses a kind of method of TEM type beta lactamases in detection breast using colloidal gold immuno-chromatography test paper strip, for detecting the beta lactamase in milk, specific preparation method is as follows:Collaurum is prepared using Frens methods, size, the uniformity, the dispersiveness of colloid gold particle are identified using ocular estimate, UV scanning, transmission electron microscope;Experiment is stabilized by MeyShi and determines most suitable antibody addition and optimum pH;Low-temperature and high-speed centrifugal process prepares gold labeling antibody, is identified using ocular estimate, UV scanning, Dot-immunogold filtration;Determine the extension rate of primary antibody, secondary antibody on detection line and nature controlling line;Screening prepares the material and treatment fluid of test strips;Line sensitivity and Detection of Stability are entered to the test strips prepared, determine minimum detection limit and holding time;The present invention is applied to the primary dcreening operation work detected on a large scale, and detection can be completed in 15min.
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