| abstract |
The present invention provides a kind of binary expression vector, the method for reduction tobacco leaf Cd accumulation and application, wherein, the binary expression vector is used for the Cd accumulation for reducing tobacco leaf, the binary expression vector includes promoter Pro NTR6 nucleotide sequence and coding OsHMA3 nucleotide sequence, and the promoter Pro NTR6 are tobacco root specific expression gene NtNTR6 promoter.Binary expression vector provided by the invention can make in tobacco leaf Cd accumulation substantially reduce by 30%, be advantageous to reduce the heavy metal cadmium content in cigarette, avoid user suck cigarette caused by Accumulation of heavy metals problem. |