http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107475262-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8261 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895 |
filingDate | 2017-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107475262-B |
titleOfInvention | A kind of gene ZmSmk3 encoding corn mTERF albumen and its cloning process and application |
abstract | The present invention relates to the cloning process of gene ZmSmk3 and the gene ZmSmk3 for encoding corn mTERF albumen a kind of, comprising the following steps: extracts the DNA of several Mutants homozygous and homozygous wildtype plant leaf respectively;The Mutants homozygous DNA of extraction is mixed into the building pond Mutants homozygous DNA;The wild type DNA of extraction is mixed into the building pond wild type DNA;Hot asymmetric interlaced PCR is carried out to the pond homozygous mutation DNA of building and the pond wild type DNA respectively, obtains PCR product segment;With the detected pcr amplification product segment of agarose gel electrophoresis, the specific band occurred in the pond Mutants homozygous DNA is recycled, carries out the sequence that sequencing obtains mutated gene ZmSmk3.The gene ZmSmk3 encodes mTERF albumen, and the mutation of the gene influences Grain Development process, and has important directive significance to breeding practice. |
priorityDate | 2017-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 420.