http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107475250-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2017-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107475250-B |
titleOfInvention | A kind of pre-treating method that intestinal contents macro genome DNA extracts |
abstract | The invention discloses the pre-treating methods that a kind of intestinal contents macro genome DNA extracts.Pre-treating method of the present invention is to be added that rotten agent is gone to clean 2 times in intestinal contents sample, add the cleaning of nucleic acid enzyme solutions, cell is cracked by freeze-thaw method, cracking process, sufficiently discharge DNA therein, intestinal contents macro genome DNA can be obtained through conventional method in protein, fiber and the RNA being eventually adding inside Proteinase K, SDS and RNA enzyme removal cell, products therefrom.Pre-treating method of the present invention has obvious effects on the extraction of the intestinal contents macro genome DNA of Omnivore, and the purity and extracted amount of extraction can meet the experiment of the downstreams such as PCR amplification, high-flux sequence, has very strong practicability. |
priorityDate | 2017-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.