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filingDate 2017-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107462534-B
titleOfInvention A system for detecting biological sulfhydryl compounds and its detection method
abstract A system for detecting biological sulfhydryl compounds and a detection method thereof. The purpose of the present invention is to provide a simple, selective and highly sensitive method for detecting biological sulfhydryl compounds. The main mechanism of this colorimetric detection is that I - can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by H 2 O 2 to generate blue oxidized TMB, with the increase of biological thiol compounds. When added, the generation of oxidized TMB is inhibited, the color of the solution becomes lighter, and the intensity of the ultraviolet-visible absorption peak at 652 nm is greatly reduced, thereby realizing the sensitive detection of biological thiol compounds. The method is not affected by other amino acids and oxidized glutathione, has good selectivity, and is successfully applied to the detection of actual samples.
priorityDate 2017-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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