http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107460186-A

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21001
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filingDate 2017-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3310c94afaa1555ecb2e7c5b65b0675a
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publicationDate 2017-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107460186-A
titleOfInvention A kind of silicone rubber immobilized chymotrypsin material and its preparation method and application
abstract The invention discloses a silicon rubber-immobilized chymotrypsin material and its preparation method and application. Using chymotrypsin as a model enzyme, undecylenoyl chloride is used to modify chymotrypsin in a phosphate buffer, and a method of sulfur-ene click chemistry is used. , uniformly mix poly[3-mercaptopropylmethylsiloxane], olefin crosslinking agent, modified chymotrypsin, and photoinitiator, and photocrosslink and immobilize under ultraviolet environment. The immobilized catalyst in the present invention can be recycled by centrifugation or filtration, has good high temperature resistance, high yield of peptide synthesis, and significantly improved stability of repeated use compared with the original enzyme. The preparation method of the invention is simple, efficient, and low in cost, and is beneficial to the industrial utilization of enzyme-catalyzed synthesis.
priorityDate 2017-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 24.