http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107459563-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-415 |
filingDate | 2017-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107459563-B |
titleOfInvention | Precipitation process in selenoprotein extraction process |
abstract | The invention discloses a precipitation process in the process of extracting selenoprotein, which is prepared by the following steps: putting the selenoprotein extract into a low-temperature reactor, reducing the temperature to be below 0 ℃ at a cooling speed of 3-5 ℃ to ensure that the selenoprotein extract is in an ice bath environment, adding glacial acetone 4 times the volume of the selenoprotein extract at the speed of 1-4 mL/min, simultaneously, introducing protective gas into the selenoprotein extraction liquid and starting a three-stage stirring program, firstly stirring for 2-3 min at the stirring speed of 1000-2000 r/min, then stirring for 4-6 min at a stirring speed of 500-800 r/min, stirring for 4-6 min at a stirring speed of 100-200 r/min, stopping ventilation after stirring, cooling to-27 to-17 ℃, and (3) placing for 3-5 h under the auxiliary treatment condition that the sound energy density of ultrasonic waves is 15-25W/mL, and drying and crushing the precipitate to obtain the selenoprotein. The method can efficiently precipitate the selenoprotein under the condition of ensuring the quality of the selenoprotein, and has wide market popularization value. |
priorityDate | 2017-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.