http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107447023-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-1434 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2017-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107447023-B |
| titleOfInvention | Method for detecting phosphorus accumulating bacteria in sewage treatment system by combining fluorescence in-situ hybridization with flow cytometry |
| abstract | A method for detecting phosphorus accumulating bacteria in a sewage treatment system by combining fluorescence in situ hybridization with flow cytometry belongs to the technical field of sewage biological treatment. The invention adopts SYBR GREEN I dye to dye the whole bacteria and marks the DNA of the whole bacteria; and hybridizing the probe PAOmix with the DNA of the phosphorus-accumulating bacteria, and marking the DNA of the phosphorus-accumulating bacteria. And (4) allowing the marked bacteria liquid to enter a flow cytometer to detect the fluorescence intensity of cells, and performing gating on the detected main flora. The position of the door displays the distribution condition of the fluorescence intensity of the target flora, and is used for analyzing the proportion of the target phosphorus-accumulating flora in the whole flora. The invention provides an economic, simple and reliable method for quantifying the phosphorus accumulating bacteria in the activated sludge of the sewage treatment system. The quantitative result of the invention is accurate and stable; the reproducibility of the experimental result is good, and the influence of personal experience and subjective judgment of an operator in the fluorescent in-situ hybridization microscopic picture quantitative method is avoided. |
| priorityDate | 2017-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.