http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107446928-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-141 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8261 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8222 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
filingDate | 2017-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-107446928-B |
titleOfInvention | Cauliflower organ development regulation miRNA sequence and application thereof |
abstract | The invention discloses a cauliflower organ development regulation miRNA sequence and a method for culturing high-plant and few-branch transgenic plants by using the same, wherein the method comprises the following steps: (1) extracting the total DNA of the cauliflower; (2) amplification of a cauliflower miRNA-1 precursor; (3) constructing and identifying a cauliflower miRNA-1 plant overexpression vector; (4) genetic transformation; (5) resistance screening and molecular identification of miRNA-1 transgenic arabidopsis; (6) phenotypic analysis of transgenic Arabidopsis thaliana over-expressed by miRNA-1. The result shows that the height of the transgenic plant is obviously higher than that of the wild type; the stem branch number of the transgenic plant is obviously less than that of the wild type; the miRNA sequence and the method for cultivating the high-plant and few-branch transgenic plants by using the miRNA sequence disclosed by the invention have important values for disclosing a regulation and control mechanism of miRNA in cauliflower organ development, further intervening the plant organ development process by adopting a genetic engineering means and cultivating high-plant and few-branch crops or economic plants meeting the breeding target. |
priorityDate | 2017-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.