http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107446928-B

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filingDate 2017-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107446928-B
titleOfInvention Cauliflower organ development regulation miRNA sequence and application thereof
abstract The invention discloses a cauliflower organ development regulation miRNA sequence and a method for culturing high-plant and few-branch transgenic plants by using the same, wherein the method comprises the following steps: (1) extracting the total DNA of the cauliflower; (2) amplification of a cauliflower miRNA-1 precursor; (3) constructing and identifying a cauliflower miRNA-1 plant overexpression vector; (4) genetic transformation; (5) resistance screening and molecular identification of miRNA-1 transgenic arabidopsis; (6) phenotypic analysis of transgenic Arabidopsis thaliana over-expressed by miRNA-1. The result shows that the height of the transgenic plant is obviously higher than that of the wild type; the stem branch number of the transgenic plant is obviously less than that of the wild type; the miRNA sequence and the method for cultivating the high-plant and few-branch transgenic plants by using the miRNA sequence disclosed by the invention have important values for disclosing a regulation and control mechanism of miRNA in cauliflower organ development, further intervening the plant organ development process by adopting a genetic engineering means and cultivating high-plant and few-branch crops or economic plants meeting the breeding target.
priorityDate 2017-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 34.