http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107446900-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y504-99016 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-12 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-12 |
| filingDate | 2015-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107446900-B |
| titleOfInvention | A kind of trehalose synthase and its preparation method and application |
| abstract | The invention discloses a kind of trehalose synthases and its preparation method and application, belong to genetic engineering and enzyme engineering field.The 289th near the trehalose synthase activated centre of Thermobifida fusca YX glutamic acid mutation is obtained mutant E289G at glycine by the present invention；By the 295th Histidine mutagenesis at asparagine mutant H295N；344th methionine is mutated into lysine mutation body M344K；367th methionine is mutated into leucine mutant M367L, and carries out double mutation on the basis of H295N and obtains mutant H295N/E289G, H295N/M344K, H295N/M367L, H295N/M344K/M367L.Even if mutant is realized containing certain glucose in substrate, the transformation efficiency that trehalose synthase prepares trehalose will not be still greatly affected, industrial value with higher. |
| priorityDate | 2015-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 364.