http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107446889-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f69c51c933a186e9b1f871a5cdd114eb |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0647 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0221 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0789 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
| filingDate | 2017-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96052ba6c14625e1889b33d0d2f5f22c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dced6d86938cf015df085797609bf7c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_038f59aa55a996e47802fea02650fc1c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e41e4c23db8416262e30d9e16c155bc9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f33472e690011b74cb627aee91725088 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41513f6d228d1ee3c3724f240b33f52c |
| publicationDate | 2017-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107446889-A |
| titleOfInvention | A kind of method of umbilical hemopoietic stem cell separation and storage |
| abstract | The invention discloses the method for a kind of separation of umbilical hemopoietic stem cell and storage, comprise the following steps：Freshly harvested bleeding of the umbilicus is taken, and addition has in the blood taking bag of anti-coagulants, and seal；HES and unconjugated bilirubin are added in pure water；Mixed liquor is crossed into resin and is sieved through filter；Bleeding of the umbilicus is slowly added into mixed liquor, stands 30 120 minutes；After standing, the plasma layer that leucocyte is contained on upper strata is collected；The blood plasma of collection is added into flushing liquor and rinses multiple, the candidate stem cell separated；Induction liquid is added into candidate stem cell, induction processing is carried out in Tissue Culture Flask, is then cultivated；Then loading storage container is put in candidate stem cell, 1 3h in ice cube, then taken out, be placed in dry ice and place 12 24h, take out afterwards, be positioned in liquid nitrogen and freeze.The present invention can isolate candidate stem cell from bleeding of the umbilicus, and carry out freezen protective, and separation and storing process are simple, easy to operate. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109589645-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109589645-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110839612-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110839612-A |
| priorityDate | 2017-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.