http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107435061-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2016-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107435061-B |
| titleOfInvention | Quantitative detection methods for mutant genes |
| abstract | The invention discloses a method for quantitative detection of mutant genes. The steps include: 1) extracting sample DNA; 2) single-strand synthesis reaction; 3) capturing and purifying microspheres; 5) use the product of step 4) as a template to carry out a single-strand synthesis reaction on the microspheres, and separate and remove the microspheres; 6) 3'-end protective exocytosis purification reaction; 7) double-end linker bridging reaction, and Purification; 8) The previous purified product was used as a template, and the single-strand synthesis reaction and the 3'-end protective exosection purification reaction were repeated; 9) Quantification, sequencing, and judgment of the mutant gene. The method uses the target gene fragment as a template to carry out repeated multi-cycle single-strand synthesis reactions, and converts the bases of a series of sites on the original template into complementary sequences, so that the target gene sequence can be converted and synthesized with maximum fidelity. After multi-stage single-strand synthesis , to obtain a sufficient amount of complete test sequences required for sequencing, thus improving the detection sensitivity and fidelity of low-proportion mutant genes. |
| priorityDate | 2016-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.