http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107428795-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1068 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 |
| filingDate | 2015-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-107428795-B |
| titleOfInvention | Methods for labeling DNA-encoding libraries |
| abstract | The present invention relates to methods for preparing encoded chemical entities. Specifically, the oligonucleotides and methods can include encoding chemical entities with wild-type linkages formed by chemical ligation techniques. The present invention relates to an oligonucleotide labeling strategy using cyanoimidazole and Zn 2+ , utilizing wild-type linkages (eg, phosphodiester linkages) derived from oligonucleotides with 5'-monophosphate and 3'- For hydroxyl-terminated oligonucleotide pairs, and also derived from 5'-hydroxyl and 3'-monophosphate ends, this strategy provides relatively high yields. This chemical ligation method is template-dependent and allows the use of orthogonal 3'- and 5'-phosphates, resulting in a high degree of sequential ligation of encoding oligonucleotides with low rates of misincorporation or miscoding control, including in the double-stranded case. |
| priorityDate | 2014-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 454.