| abstract |
The invention discloses one kind, from Methylobacter, (Methylobacterium sp. are in the resource number of Chinese Bei Na DSMZs：BNCC195932 gene order of Acetoacetyl-CoA reductase (PhaB)) and its preparation method and application, i.e. design primer amplifies the nucleotide sequence of the gene from the bacterium, again by the gene cloning of the enzyme to coli expression carrier, obtain can the heterogenous expression enzyme E. coli recombinant stain, the Acetoacetyl-CoA reductase prepared with the bacterial strain heterogenous expression, can be used for synthesizing polyhydroxyalkanoateby (PHA) and hydroxy fatty acid. |