| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f97f0fa258a008bf056ad04b7f380dda |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-55 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21004
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6427 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 |
| filingDate |
2015-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21b30d1cd9bff9f121abd11191304483
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d0d3e565f3c6eaf94f672fef83c25ba |
| publicationDate |
2017-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-107406868-A |
| titleOfInvention |
Method for obtaining peptide fragments from antibodies by protease hydrolysis reaction with limited reaction field |
| abstract |
The present invention provides a method for increasing the yield of a peptide fragment comprising the Fab region or variable region of the antibody, or a part thereof, derived from a target monoclonal antibody, and a kit used in the method. The method is characterized in that it includes the following steps: for the target monoclonal antibody immobilized on the inner surface of the pores of the porous body and the protease immobilized on the surface of the nanoparticle so that they are uniformly dispersed in the liquid Stirring at a low speed lower than 100rpm or tapping and rotating to make them contact, thereby performing the digestion of the aforementioned target monoclonal antibody, and the nanoparticles have an average particle diameter greater than the average pore diameter of the aforementioned porous body; wherein, The average pore diameter of the porous body is in the range of 10 nm to 200 nm, and the average particle diameter of the nanoparticles is in the range of 50 nm to 500 nm. |
| priorityDate |
2015-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |