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publicationDate 2017-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-107384797-A
titleOfInvention The separation method of giant panda enteron aisle actinomyces
abstract The invention belongs to field of microbial culture technology, specially a kind of separation method of giant panda enteron aisle actinomyces, the fresh giant panda fecal specimens of collection are added into water, add phenol, Tween 20, CMC, culture and centrifugal force, supernatant dilution, obtain bacteria suspension, bacteria suspension is coated on actinomyces isolation medium and cultivated, and the doubtful actinomyces bacterium colony of picking is in ISP 4 Rule and purify on culture medium, be inoculated in ISP 4 On slant medium.The present invention carries, Escherichia coli high to giant panda excrement water content and some other Gram-negative bacteria is more, actinomyces the features such as proportion is relatively low in giant panda enteron aisle, and phenol is added in sample pre-treatments to suppress the growth of general bacterium;The addition of CMC and polysorbas20 can dispersed sample to greatest extent, fully discharge actinomyces, improve the separating effect of later stage actinomyces.The present invention helps more comprehensively to understand the population composition of actinomyces in giant panda enteron aisle, further enriches the resource of giant panda enteron aisle actinomyces.
priorityDate 2017-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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