abstract |
The present invention provides a kind of extracting method of water father-in-law active ingredient, belongs to field of plant extraction, comprises the following steps:Raw material is used as using the fruit of water father-in-law and bud first, after being cleaned it is clean after, naturally dry at room temperature, water father-in-law fruit is beaten into pulp under specified conditions, and handled in ultrasonic environment, after extraction is mixed with the active liquid of self-control in homogenizer, it is then centrifuged for isolated supernatant a and solid content, The Bud of Cleistocalyx Operculatus and the solid content are extracted by the way of microwave abstracting again, supernatant b is centrifuged after extraction again, finally by the supernatant a, it is put into the chromatographic column of macroreticular resin and is eluted after b mixing, after being concentrated under reduced pressure by gained eluent and reclaim ethanol, be dried in vacuo and produce the water father-in-law active ingredient, the present invention can improve the recovery rate of water father-in-law's active ingredient. |