abstract |
The present invention provides a kind of primer, kit and its application and authentication method for being used to identify attack tea snail, belongs to biological technical field, and primer includes having SEQ ID NO:The sense primer of nucleotide sequence shown in 1 and there is SEQ ID NO:The anti-sense primer of nucleotide sequence shown in 2.During use, enter performing PCR amplification using the DNA of primer pair tea snail to be detected, and establish the second biosystem tree, by being contrasted with the first biosystem tree, if snail to be detected forms monosystem group with known attack tea snail, it then can determine whether that tea snail to be detected is fast and simple for attack tea snail, the authentication method. |